Korean J Intern Med. 2023;38(1):27-38.

Analysis of the gut microbiome using extracellular vesicles in the urine of patients with colorectal cancer.

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제1저자 윤혁, 김남은 · 교신저자 박영수

PMID 36353788 PMC9816683 DOI 10.3904/kjim.2022.112 IF 2.2 소화기내과

소속 (5)

Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Department of Public Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Korea.
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND/AIMS We evaluated the gut microbiome using extracellular vesicles (EVs) in the urine of patients with colorectal cancer (CRC) to determine whether gut-microbe-derived EVs could be a potential biomarker for the diagnosis of CRC.

METHODS EVs were isolated from the urine of patients with CRC and healthy controls. DNA was extracted from the EVs, and the bacterial composition was analyzed using next-generation sequencing of the 16S rRNA.

RESULTS A total of 91 patients with CRC and 116 healthy controls were enrolled. We found some specific microbiomes that were more or less abundant in the CRC group than in the control group. The alpha-diversity of the gut microbiome was significantly lower in the CRC group than in the control group. A significant difference was observed in the beta-diversity between the groups. The alpha-diversity indices between patients with early- and late-stage CRC showed conflicting results; however, there was no significant difference in the beta-diversity according to the stage of CRC. There was no difference in the alpha- and beta-diversity of the gut microbiome corresponding to the location of CRC (proximal vs. distal).

CONCLUSION A distinct gut microbiome is reflected in the urine EVs of patients with CRC compared with that in the healthy controls. Microbial signatures from EVs in urine could serve as potential biomarkers for the diagnosis of CRC.

Keywords

Colorectal neoplasmsExtracellular vesiclesMicrobial diversityMicrobiotaUrine

Figures

**Figure 1** Taxonomic composition (A–D) and principal coordinate analysis (E–H) of the gut microbiome derived from the urine extracellular vesicles of patients with colorectal cancer and healthy controls. (A) Phylum level, (B) family level, (C) genus level, (D) alpha-diversity, (E) UniFrac distance, (F) weighted-UniFrac distance, (G) Bray-Curtis dissimilarity, and (H) Jacaard distance. CRC, colorectal cancer; OTU, operational taxonomic unit. ^a p ≤ 0.0001.

**Figure 2** Taxonomic composition (A–D) and principal coordinate analysis (E–H) of the gut microbiome derived from the urine extracellular vesicles of patients with early and late colorectal cancer. (A) Phylum level, (B) family level, (C) genus level, (D) alpha-diversity, (E) UniFrac distance, (F) weighted-UniFrac distance, (G) Bray-Curtis dissimilarity, and (H) Jacaard distance. CRC, colorectal cancer; OTU, operational taxonomic unit. ^a p ≤ 0.05.

**Figure 3** Taxonomic composition (A–D) and principal coordinate analysis (E–H) of the gut microbiome derived from the urine extracellular vesicles of patients with proximal and distal colorectal cancer. (A) Phylum level, (B) family level, (C) genus level, (D) alpha-diversity, (E) UniFrac distance, (F) weighted-UniFrac distance, (G) Bray-Curtis dissimilarity, and (H) Jacaard distance. CRC, colorectal cancer; OTU, operational taxonomic unit.

그림 출처: PubMed Central 공개 논문 (PMC9816683)

Conflict of interest statement

Conflict of interest . No potential conflict of interest relevant to this article was reported.

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