J Korean Med Sci. 2016;31(2):315-20.

Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.

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제1저자 박완범 · 교신저자 오명돈

PMID 26839489 PMC4729515 DOI 10.3346/jkms.2016.31.2.315 IF 3.2 감염내과

소속 (5)

Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
Macrogen, Seoul, Korea.
Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.

Abstract

During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

Keywords

KoreaMicroscopy, ElectronMiddle East Respiratory SyndromeMiddle East Respiratory Syndrome CoronavirusPhylogeny

Figures

**Fig. 1** Cytopathic effects of MERS-CoV in Vero cell cultures and Electron microscopy image of MERS-CoV. Vero cells were inoculated with oropharyngeal swab sample. (A) Vero cell cultures in negative control. (B) Cytopathic effects (rounding and detachment of cells) in Vero cell cultures 3 days after inoculation of the sample. (**C, D**) Transmission electron microscopy image of Vero cells infected with MERS-CoV. White arrow denotes nuclear membrane. Brown scale bar indicates 100 μm (A and B). Black scale bar indicates 500 nm (C) and white scale bar does 200 nm (D).

**Fig. 2** Molecular phylogenetic analysis. Phylogenetic tree on complete genome (A), S genes (B), and ORF1ab genes (C) for the 101 gene sequences of MERS-CoV. The evolutionary history was inferred by using the maximum likelihood method based on the Tamura-Nei model (16). Evolutionary analyses were conducted in MEGA6 (17). Red box indicates our virus isolate.

그림 출처: PubMed Central 공개 논문 (PMC4729515)

Conflict of interest statement

DISCLOSURE: The authors have no potential conflicts of interest to disclose.

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