Am J Trop Med Hyg. 2010;83(4):930-5.
Antigenic drift of Orientia tsutsugamushi in South Korea as identified by the sequence analysis of a 56-kDa protein-encoding gene.
제1저자 박상원 · 교신저자 이창섭
소속 (1)
- Department of Internal Medicine, Seoul National University College of Medicine and Boramae Medical Center, Seoul, Republic of Korea. hswon1@snu.ac.kr
Abstract
This study was performed to determine the nationwide antigenic diversity of Orientia tsutsugamushi in South Korea. Sequence analysis was performed around variable domains I and II of a 56-kDa protein-encoding gene. We used eschar to overcome the disadvantages of conventional serotyping. The serological passive hemagglutination assay (PHA) was assessed based on the genotyping results. We analyzed 153 isolates from scrub typhus patients in major endemic areas and found that Boryong was the major strain (68.6%). New strains were also identified: Taguchi (19.6%), Kanda/Kawasaki (9.2%), and UAP7 (1.3%). PHA yielded significantly fewer positive results among Kawasaki strains (P < 0.001), which are not included in the PHA antigen panel. In South Korea, Boryong was still the predominant strain, but the sequence analysis identified new changes in minor strains (30.1%). This antigenic drift had a negative effect on the PHA results. Periodic surveillance of the contemporary strains using sequence analysis is needed.
그 밖의 정보
- 출판 형태
- Journal Article, Research Support, Non-U.S. Gov't
- 저널
- The American journal of tropical medicine and hygiene · eISSN 1476-1645
- MeSH
- Adolescent · Adult · Aged · Aged, 80 and over · Antigenic Variation / genetics · Antigens, Bacterial / genetics · Bacterial Proteins / genetics · Female · Genetic Drift* · Genotype · Hemagglutination Tests · Humans · Male · Middle Aged · Molecular Sequence Data · Orientia tsutsugamushi / genetics / immunology · Phylogeny · Phytohemagglutinins / blood · Polymerase Chain Reaction · Prospective Studies · Republic of Korea / epidemiology · Scrub Typhus / epidemiology / microbiology · Sequence Alignment · Sequence Analysis, DNA · Young Adult
- Substances
- Antigens, Bacterial · Bacterial Proteins · Phytohemagglutinins
- 데이터
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- 참고문헌
- 28편