Gut Liver. 2021;15(1):70-76.

Expression of Chemokine CCL28 in Ulcerative Colitis Patients.

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제1저자 이동석 · 교신저자 김지원

PMID 32102131 PMC7817927 DOI 10.5009/gnl19273 IF 4.1 소화기내과

소속 (2)

Departments of Gastroenterology, SMG-SNU Boramae Medical Center, Seoul National University of College of Medicine, Seoul, Korea.
Departments of Laboratory Medicine, SMG-SNU Boramae Medical Center, Seoul National University of College of Medicine, Seoul, Korea.

Abstract

BACKGROUND/AIMS Ulcerative colitis (UC) is an inflammatory bowel disease for which new serological markers are required. The purpose of this study was to assess the role of the mucosa-associated epithelial chemokine CCL28 in UC.

METHODS The study included 50 patients; of these, 25 were patients with UC, and 25 were healthy controls. The levels of serum CCL28 were analyzed using enzyme-linked immunosorbent assay. CCL28 expression was analyzed by immunohistochemistry (IHC) in 15 representative colon tissues biopsied based on disease activity (UC patients with severe activity, five samples; UC patients with mild activity, five samples; healthy controls, five samples).

RESULTS The serum CCL28 levels were remarkably higher (p<0.05) in patients with UC (median, 235.7 pg/mL; IQR, 63.8 to 117.2 pg/mL) than in healthy controls (median, 48.9, pg/mL; IQR, 35.9 to 42.0 pg/mL). However, there was no significant difference in serum CCL28 according to disease extent or activity. In contrast, IHC analysis revealed a significant difference in CCL28 consistent with disease status, disease extent, and disease activity.

CONCLUSIONS CCL28 could be useful for diagnosing UC. However, further validations of CCL28 on disease activity and severity are needed.

Keywords

BiomarkerCCL28ColitisDisease activitySerologic markersulcerative

Figures

**Fig. 1** Serum CCL28 levels in patients with ulcerative colitis (n=25) and healthy controls (n=25). *p<0.05, versus controls.

**Fig. 2** Serum CCL28 levels in ulcerative colitis patients with (n=17) and without (n=8) pancolitis.

**Fig. 3** Serum CCL28 levels in ulcerative colitis patients with severe disease activity (n=5), moderate disease activity (n=10), and mild disease activity (n=10).

**Fig. 4** Representative images of immunohistochemistry (IHC) analysis of CCL28 in mucosal tissue extracted from colon biopsy samples. (A) Sample from an ulcerative colitis (UC) patient with severe disease activity. The mean IHC score±SD of all samples from this group (n=5) was 3±0 (×100). (B) Sample from a UC patient with mild disease activity. The mean IHC score±SD of all samples from this group (n=5) was 0.80±0.84 (×100). (C) Sample from a normal tissue obtained from a healthy control. The mean IHC score±SD of all samples from this group (n=5) was 0±0 (×100).

**Fig. 5** Receiver operating characteristic curve analysis of serum CCL28 for the diagnosis of ulcerative colitis. The sensitivity was 96% and specificity was 92%. The gray line is the reference line and the black line is the receiver operating characteristic (ROC) curve. AUC, area under the ROC curve.

그림 출처: PubMed Central 공개 논문 (PMC7817927)

Conflict of interest statement

CONFLICTS OF INTEREST. No potential conflict of interest relevant to this article was reported.

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