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Int J Infect Dis. 2018;72:8-10.

Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection.

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제1저자 박완범,Poon LLM · 교신저자 오명돈,Peiris M

PMID 29753119 PMC7110884 DOI 10.1016/j.ijid.2018.05.003 IF 4.6 감염내과
소속 (6)
  1. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea; Laboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South Korea.
  2. School of Public Health, The University of Hong Kong, Pokfulam, Hong Kong, China.
  3. Laboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South Korea.
  4. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea.
  5. School of Public Health, The University of Hong Kong, Pokfulam, Hong Kong, China. Electronic address: malik@hku.hk.
  6. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea; Laboratory of Infection and Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, South Korea. Electronic address: mdohmd@snu.ac.kr.

Abstract

BACKGROUND Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control. The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative. This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens.

METHODS Upper and lower respiratory samples were obtained from 17 MERS-CoV-infected patients. MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR.

RESULTS In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA. In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate.

CONCLUSIONS These findings suggest that MERS-CoV does not replicate well in the upper respiratory tract.

Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

Keywords

Coronavirus infectionsMessengerRNARespiratory system

Figures

Figure 1
Figure 1 Detection of Middle East respiratory syndrome coronavirus (MERS-CoV) sub-genomic mRNA in (A) sputum and transtracheal aspirates; (B) throat swabs; (C) nasopharyngeal swabs. A pink dot denotes a positive RT-PCR result for MERS-CoV sub-genomic mRNA, while a gray dot indicates a negative result. The blue lines indicate the duration from symptom onset to the last positive result for MERS-CoV genomic RNA. Patients A–I were in the severe group and patients J–Q were in the mild group. Among the severe group subjects, patients A–E received ventilator therapy, while patients F–I did not. 5UTR-S and 5UTR-N denote the sub-genomic mRNA of spike protein and nucleocapsid protein, respectively.
Figure 2
Figure 2 Middle East respiratory syndrome coronavirus (MERS-CoV) genomic RNA (upE) titers in sputum and transtracheal aspirates with vs. without sub-genomic mRNA detection. Solid lines indicate the mean and standard error of the mean. The dashed line indicates the detection limit.

그림 출처: PubMed Central 공개 논문 (PMC7110884)

그 밖의 정보
출판 형태
Journal Article
저널
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · eISSN 1878-3511
전자 출판
2018-05-09
MeSH
Coronavirus Infections / physiopathology / virology · Humans · Middle East Respiratory Syndrome Coronavirus / isolation & purification · Real-Time Polymerase Chain Reaction · Respiratory System / physiopathology / virology · Reverse Transcription · Sputum / virology · Virus Replication* · Virus Shedding*
참고문헌
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